diff --git a/clang/bindings/python/tests/cindex/test_translation_unit.py b/clang/bindings/python/tests/cindex/test_translation_unit.py
--- a/clang/bindings/python/tests/cindex/test_translation_unit.py
+++ b/clang/bindings/python/tests/cindex/test_translation_unit.py
@@ -140,7 +140,7 @@
         h3 = os.path.join(kInputsDir, "header3.h")
         inc = [(src, h1), (h1, h3), (src, h2), (h2, h3)]
 
-        tu = TranslationUnit.from_source(src)
+        tu = TranslationUnit.from_source(src, ["--no-default-config"])
         for i in zip(inc, tu.get_includes()):
             eq(i[0], i[1])
 
@@ -151,7 +151,7 @@
         h3 = os.path.join(kInputsDir, "header3.h")
         inc = [h1, h3, h2, h3, h1]
 
-        tu = TranslationUnit.from_source(src, options=TranslationUnit.PARSE_DETAILED_PROCESSING_RECORD)
+        tu = TranslationUnit.from_source(src, ["--no-default-config"], options=TranslationUnit.PARSE_DETAILED_PROCESSING_RECORD)
         inclusion_directive_files = [c.get_included_file().name for c in tu.cursor.get_children() if c.kind == CursorKind.INCLUSION_DIRECTIVE]
         for i in zip(inc, inclusion_directive_files):
             self.assert_normpaths_equal(i[0], i[1])